ANN ARBOR, MI - 01/16/2019 (PRDistribution.com)
(ANN ARBOR, Mich. – January 16, 2019) Swift Biosciences today announced the launch of its Swift Amplicon 16S+ITS Panel, facilitating routine NGS analysis of complex microbial samples (e.g. bacteria, archaea, fungi) using a single, multiplexed primer pool targeting the 16S rRNA gene (all variable regions, V1-V9) and ITS1 and ITS2 for fungi. With a comprehensive yet targeted library approach in a single-tube assay, the panel enriches the sequences used for identifying the structure of a microbial community in a format optimized for NGS.
“This kit will save costs and time, and it produces better data than homebrew and other available 16S methods” said Drew McUsic, PhD, Swift’s Product Manager. “Our amplicon chemistry generates excellent sequencing quality without the need for PhiX spike-ins and shows equivalent performance with 2x150 reads vs. 2x300. In addition, by leveraging all 16S rRNA gene variable regions, this method maximizes sensitivity to detecting the presence and relative abundance of various community members.”
This new product includes primers, PCR reagents, and dual indexed adapters for converting the genetic material of a metagenomic community into Illumina®-compatible libraries in a 2-hour workflow.
The Swift Amplicon 16S+ITS Panel is currently available in a 48-reaction kit size, with 8x12 combinatorial dual indexing for multiplexing up to 96 samples per MiSeq® run. Data may be processed using commonly available tools including the Illumina® BaseSpace® 16S Metagenomics and ITS Metagenomics app, as well as open-source tools such as RDPtools and MOTHUR. A 96-reaction kit with indexed adapters for multiplexing up to 384 samples will be available soon for higher throughput users.
Applications and Sample Types:
For more information, visit SwiftBioSci.com and follow Swift Biosciences on Twitter (@SwiftBioSci)